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Demo User profile
| stats | karma | 20 |
| votes | 0 0 | |
| member since | 5/3/18, 2:49 PM | |
| last connection | 5/7/24, 5:08 PM | |
| bio | website | |
| location |
Namur (Loyers)
,
Belgium
|
Karma
| Supporter | 1 awarded users | First upvote |
| Autobiographer | 2 awarded users | Completed own biography |
隨時(shí)了解最新的技術(shù)快訊
關(guān)于這個(gè)技術(shù)問(wèn)答社區(qū)
CRISPR / Cas9系統(tǒng)是用于基因編輯的RNA引導(dǎo)核酸內(nèi)切酶技術(shù)。該系統(tǒng)需要包含與PAM（Protospacer Adjacent Motif）位點(diǎn)相鄰的20bp靶序列的指導(dǎo)RNA（gRNA），以將Cas9核酸內(nèi)切酶引導(dǎo)至切割位點(diǎn)。Geneious中的Find CRISPR網(wǎng)站工具將搜索所選基因中的gRNA（“CRISPR”）位點(diǎn)，并在您感興趣的基因組中搜索脫靶結(jié)合位點(diǎn)。選定序列中的每個(gè)CRISPR站點(diǎn)根據(jù)它可能會(huì)綁定到多少個(gè)脫離目標(biāo)站點(diǎn)以及脫離目標(biāo)站點(diǎn)與原始序列的相似程度進(jìn)行評(píng)分。
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